Wen Liang Chen

En-Chu-Kong Hospital, Taipei, Taiwan

Post-Doctor fellow, En-Chu-Kong hospital Common Laboratory

2008/4-Present

National Yang Ming University, Taipei, Taiwan

Post-Doctor fellow, Institute of Emergency and Critical Care Medicine

2007/11-2008/3

China Institute of Technology, Taipei, Taiwan

Assistant Professor, Department of Biological Science and Technology

2008-Present

National Science Council Research project (延攬博士後研究人才) Establishment of proteomic mapping in non-small cell lung carcinoma. 建立非小型細胞肺癌之蛋白質圖譜計劃編碼: 096-2811-B-010-005- NT$1,600,000	2007/7-2009/7
National Science Council Research project (Co-PI) Establishment of proteomic mapping in non-small cell lung carcinoma. 建立非小型細胞肺癌之蛋白質圖譜計劃編碼: 96-2628-B-010-004-MY2 NT$1,400,000	2007/7-2009/7
National Science Council Research project (Co-PI) The mechanism mediating actions of nm23-H1 on metastasis and chemosensitivity in esophageal cancer nm23-H1於食道癌中影響腫瘤轉移及化療藥物之機轉計劃編碼: 96-2314-B-010-010-MY2 NT$1,000,000	2007/7-2009/7
Long-Ren Cultural and Educational Foundation Research project (Co-PI) Development of a specific monoclonal antibody for detection of tumor metastasis in non-small cell lung cancer. 製作單株抗體專一性辨識非小型細胞肺癌研究癌細胞增生、轉移及侵犯 NT$500,000	2008/1-2008/12
En-Chu-Kong hospital Research project (Co-PI) Anti-inflammatory effect of Cortistatin on non-small cell lung adenocarcinoma - an emphasis on tumor cell proliferation and invasion. 探討Cortistatin對抗發炎反應及非小型細胞肺癌細胞之影響 NT$1,000,000	2008/7-2009/7
En-Chu-Kong hospital Research project (Co-PI) The effect of cortistatin on angiogenesis in esophageal squqamous cell carcinoma. 探討Cortistatin對食道癌細胞血管新生因子之影響 NT$1,000,000	2008/7-2009/7

1. Liau CY, Chang TM, Pan JP, Chen WL, Mao SJT. Purification of human plasma haptoglobin by hemoglobin-affinity column chromatography. J Chromatogr B. 2003, 25;790:209-216. (IF = 2.18, Subject categories: 19 / 70) (SCI)

2. Chen WL, Huang MT, Liu HC, Li CW, Mao SJT. Distinction between dry and raw milk using monoclonal antibodies prepared against dry milk proteins. J Dairy Sci. 2004, 87:2720-2729. (IF = 2.13, Subject categories: 8 / 94) (SCI)

3. Song CY, Chen WL, Yang MC, Huang JP, Mao SJT. Epitope mapping of a monoclonal antibody specific to bovine dry milk: involvement of residues 66-76 of strand D in thermal denatured beta-lactoglobulin. J Biol Chem. 2005, 280:3574-3582. (IF = 6.36, Subject categories: 31 / 261) (SCI)

4. Chen WL, Huang MT, Liau CY, Ho JC, Hong KC, Mao SJT. {beta}-Lactoglobulin is a Thermal Marker in Processed Milk as Studied by Electrophoresis and Circular Dichroic Spectra. J Dairy Sci. 2005, 88:1618-1630. (IF = 2.13, Subject categories: 8 / 94) (SCI)

5. Chen WL, Liu WT, Yang MC, Huang MT, Mao SJT. A novel conformation-dependent monoclonal antibody specific to native structure of β-lactoglobulin and its application. J Dairy Sci. 2006, 89:912-921. (IF = 2.13, Subject categories: 8 / 94) (SCI)

6. Liu HC, Chen WL, and Mao SJT. Antioxidant nature of bovine milk β-lactoglobulin. J Dairy Sci. 2007, 90:547-515. (IF = 2.13, Subject categories: 8 / 94) (SCI)

7. Yueh CH, Lai YA, Chen WL, Hsu HH, Mao SJT. Improved method for haptoglobin 1-1, 2-1, 2-2 purification using monoclonal antibody affinity chromatography in the presence of sodium dodecyl sulfate. 2007, 845:210-217. J Chromatogr B. (IF = 2.18, Subject categories: 19 / 70) (SCI)

8. Yang MC, Guan HH, Liu MY, Lin YH, Yang JM, Chen WL, Chen CJ, and Mao SJT. Crystal structure of a secondary vitamin D3 binding site of milk beta-lactoglobulin. 2008; 71:1197-1210. Proteins: Structure, Function, and Bioinformatics. (IF = 3.7, Subject categories: 12 / 66) (SCI)

9. Yang MC, Guan HH, Liu MY, Yang JM, Chen WL, and Mao SJT. Crystal structure of a secondary vitamin D3 binding site of milk beta-lactoglobulin. PDB structure, NO. 2GJ5. (SCI)

10. Chen WL, Liu WT, Yang MC, Huang MT, Mao SJT. β-lactoglobulin plays a provocative role in lymphocyte proliferation. J Dairy Sci. (To be Submitted) (SCI)

11. Hsu HH, Yueh CH, Chen WL, Huang PY, Mao SJT. Structure of human plasma haptoglobin a1 probed by a unique monoclonal antibody. (Manuscript in preparation)

12. 陳文亮 及毛仁淡；牛乳加熱前與加熱後乳球蛋白之差異；科學新天地；龍騰文化出版社；2005；10；p64-67。

13. 陳文亮, 及毛仁淡; Gene expression microarray analysis在癌症生物學、藥理學和藥物研發的應用; 越吟出版社; 電子月刊; 2002; 88; p76-p80

CONFERENCE PAPER

1. Chen WL, and Mao SJT. (2000) A simple method for the determination of plasma fibrinogen using a microtiter plate. The fifteenth joint annual conference of biomedical sciences. Programs & abstracts. P232

2. Chen WL, Ho JC, Hoang KC, and Mao SJT. (2001) Differentiation between fresh and powdered milk using chemical, physical, and immunochemical analyses. The sixteenth joint annual conference of biomedical sciences. Programs & abstracts.

3. Chen WL, Mao SJT. (2002) Analyses of β-lactoglobulin as a thermal marker in raw and commercially processed milk using a native gel electrophoresis and circular dichroic spectrum. Experimental Biology 2002 New Orleans, LA, USA. FASEB J. 16:A1187.

4. Ko YC, Chen WL, Liau CY, Kao WS, and Mao SJT. (2002) Preparation of colloidal gold for immunoassay. A simple and stabilized procedure. The seventeenth joint annual conference of biomedical sciences. Oral presentation.

5. Liau CY, Chen WL, Wu YJ, Lin CC, and Mao SJT. (2002) Differentiation of Milk Proteins Using Phage Displayed Antibodies. The seventeenth joint annual conference of biomedical sciences. Oral presentation.

6. Chen WL, Mao SJT. (2003) Distinguish between dry and raw milk using monoclonal antibodies prepared against dry milk proteins: LG is a sensitive thermal marker in dry milk. Experimental Biology 2003 San Diego, USA. FASEB J. 17:A1127.

7. Mao SJT, Liau CY, Chang TM, Pan RB, Chen WL. (2003) Purification of human plasma haptoglobin by hemoglobin affinity chromatography. Experimental Biology 2003 San Diego, USA. FASEB J. 17:A999.

8. Chen WL, Huang MT, Liu HC, Li CW, Mao SJT. (2003) Production of conformationally dependent monoclonal antibody against thermal-denatured milk. The eighteenth joint annual conference of biomedical sciences. Oral presentation. P81

9. Liu HC, Huang MT, Chen WL, Mao SJT. (2003) Effect of disulfide bond for the antioxidant activity of β-lactoglobulin. The eighth conference on biochemical engineering. Oral presentation. P205

10. Song CY, Chen WL, Wang SS, Mao SJT. (2004) Thermal denaturation of milk β-lactoglobulin. The nineteenth joint annual conference of biomedical sciences. Programs & abstracts. P

11. Mao SJT, Chen WL, Tseng CF, Li CW, Wu IJ, Chen YM. (2004) Rapid immunoassay on the detection of antibody or SARS-CoV in patients with SARS. Progress Report Meeting of SARS Research Project in Taiwan. Oral Presentation.

12. Chen WL, Huang MT, Li CW, Liu HC, Mao SJT. (2004) Thermal denaturation of β-lactoglobulin as probed by an monoclonal antibody. Experimental Biology 2004 Washington, DC, USA. FASEB. 18:A144.

13. Chen WL, Yang MC, Song CY, Mao SJT. (2005) Thermal denaturation ofβ-lactoglobulin as probed by specific monoclonal antibodies. The twentieth joint annual conference of biomedical sciences. Programs & abstracts. P396

14. Yang MC, Chen WL, Mao SJT. (2005) Ligand binding ability of β-lactoglobulin in native and thermal denatured state. The twentieth joint annual conference of biomedical sciences. Oral presentation. P230

15. Chen W L, Huang MT, and S. J. T. Mao. (2005) A novel two-dimentional gel electrophoresis for studing the cress-linking between β-Lactoglobulin and milk proteins. 2005 ADSA-ASAS-CSA joint annual meeting. Cincinnati, OH. Programs & abstracts. P144.

16. S. J. T. Mao, Chen WL, Yang MC, and Liu WT. Distinguish between native and thermally denatured β-lactoglobulin using a monoclonal antibody as a probe. 2005 ADSA-ASAS-CSA joint annual meeting. Cincinnati, OH. Oral presentation. P77.

17. Chen WL, Liu WT, Yang MC, Huang MT, Mao SJT. (2006) β-lactoglobulin plays a provocative role in lymphocyte proliferation. Fourteenth Symposium on Recent Advances in Cellular and Molecular Biology. Programs & abstracts. P159

18. Chen WL, Liu WT, Yang MC, Huang MT, Mao SJT. (2006) β-lactoglobulin plays a provocative role in lymphocyte proliferation. Experimental Biology 2006. San Francisco, CA. Programs & abstracts.

19. Chen WL, Liu WT, Yang MC, Huang MT, Mao SJT. (2006) β-lactoglobulin plays a provocative role in lymphocyte proliferation. The twenty-first joint annual conference of biomedical sciences. Programs & abstracts. P120.

20. Yueh CH, Chen WL, Huang PC, Mao SJT. (2006) Development of a two epitope immunoassay in measuring free PSA and PSA-ACT complex for prostate cancer. The twenty-first joint annual conference of biomedical sciences. Oral presentation. P68.

22. Yueh CH, Lai YA, Hsu HH, Chen WL, Mao SJT. (2006) Improved method for haptoglobin 1-1, 2-1, 2-2 purification using monoclonal antibody affinity chromatography in the presence of sodium dodecyl sulfate. 10^th International Symposium on Biochromatography. Lille, France. Programs & abstracts.

23. Chen WL, Yang LL, Chao LS, Wang LS. (2008) Differential analysis of two-dimension gel electrophoresis profiles of five human non small cell lung carcinoma. Sixteenth Symposium on Recent Advances in Cellular and Molecular Biology. Programs & abstracts. P168.

24. Chen WL, Yang LL, Chao LS, Wang LS. (2008) Differential analysis of two-dimension gel electrophoresis profiles of five human non small cell lung carcinoma. The 23^rd joint annual conference of biomedical sciences. Programs & abstracts.

25. Chen WL, Yang LL, Chao LS, Wang LS. (2008) Cytochrome c oxidase subunit Va is a new marker in non small cell lung carcinoma, 2008 TPS Eastern International Conference. Programs & abstracts. P62.

Ph.D.: College of Biological Science and Technology, National Chiao Tung University	2001-2006
B.S.: Department of Animal Science, Tung Hai University	1996-2000