National Chiao Tung University

Lab of Molecular Genetics

 

PIYun-Liang Yang, Ph.D.

TEL:(03)5712121#56920


   ◎ National Chung-Hsing University Department of Plant Pathology. B.S.

    Indiana University, Bloomington, in Program of Molecular, Cellular & Developmental Biology. Ph.D.

    Indiana University, Bloomington. Postdoctoral Research Associate

    Harvard Medical School, BWH. Medical Research Fellow

   ◎ National Chiao Tung University, Hsin Chu. Assistant Professor

    ◎ National Chiao Tung University, Hsin Chu. Associate Professor

   ◎ National Chiao Tung University, Hsin Chu. Professor



§ Curriculum:  

      General Biology

    General Biology Laboratory

    Genetics

      Molecular Biology

    Virology

    Molecular Mycology


§ Research interests

The interest of the laboratory is to study the microbial pathogenesis from the angle of molecular genetics & biochemistry. Our current models are pathogenic fungus, Candida & Flavivirus. In addition to academic research, we would like ultilize the information obtained in our study for Biomedical field.

At the present, there are three major topics in the lab:

A. Molecular Pathogenesis:

We employ function genetics study to obtain microbial virulence genes in order to understand the pathogenic
       interaction between microbes & host cells. Model organisms: Candida albicans, Dengue virus.

B. Mechanism of drug-resistance:

                   The emergence of drug-resistant fungi has become an issue in clinical medicine. We study the gene regulation  
         of drug-resistance in Candida.

   C. Molecular Biology of flavivirus

 


§ Publication List:  


Hsiu-Jung Lo, Kuo-Yun Tseng, Yeong-Yi Kao, Ming-Yang Tsao, Han-Lun Lo, Yun-Liang Yang*.  (2015).  Cph1p negatively regulating MDR1 involved in drug resistance in Candida albicans.  International Journal of Antimicrobial Agents.  Accepted.  SCI

Hsiu-Jung Lo, Wen-Li Chu, Ci-Hong Liou, Szu-Hsuan Huang, Kay-Hooi Khoo, Yun-Liang Yang*. (2015).  Ndt80p is involved in L-sorbose utilization through regulating SOU1 in Candida albicans. International Journal of Medical Microbiology.  Accepted.  SCI.

Yun-Liang Yang, Wen-Li Chu, Chih-Chao Lin, Sheng-Hua Tsai, Pe-Tin Chang, Hsiu-Jung Lo.  (2014).  An Emerging Issue of Mixed Yeast Cultures.  Journal of Microbiology, Immunology and Infection 47:339-344.   SCI.

Mao-Wang Ho, Yun-Liang Yang, Chih-Chao Lin, Chih-Yu Chi, Hui-Ting Chen, Po-Chang Lin, Li-Yun Hsieh, Chia-Hui Chou, Wen-Li Chu, Chiou-Pyng Wu, Tsia-Ling Lauderdale, Hsiu-Jung Lo.  (2014 June).  Yeast Oropharyngeal Colonization in Human Immunodeficiency Virus-infected Patients in Central Taiwan.  Mycopathologia 177:309-317.  SCI.

Te-Pin Chang, Mao-Wang Ho, Yun-Liang Yang, Pai-Chang Lo, Pei-Sheng Lin, An-Huei Wang, Hsiu-Jung Lo.  (2013 December).  Distribution and Drug Susceptibilities of Candida Species Causing Candidemia from a Medical Center in Central Taiwan.  Journal of Infection and Chemotherapy 19:1065-1071.  SCI.

Yun-Liang Yang, Yi-Wen Liu, Hui-Ting Chen, Moan-Shane Tsai, Wen-Li Chu, Hsiu-Jung Lo.  (2013 November).  Genotype Analysis Based on Intergenic Spacer 1 Sequences of Trichosporon asahii collected in Taiwan.  Medical Mycology 51: 880-803.  SCI.

Yun-Liang Yang, Hui-Ting Chen, Chih-Chao Lin, Wen-Li Chu, Hsiu-Jung Lo.  (2013).  
     Species Distribution and Drug Susceptibilities of Candida Isolates in TSARY 2010.
     Diagnostic Microbiology and Infectious Disease 76: 182-186.  (June).  SCI.

Hui-Ching Ko, Ting-Yin Hsiao, Chiung-Tong Chen, and Yun-Liang Yang*.  (2013).  Candida albicans ENO1 Null Mutants Exhibit Altered Drug Susceptibility, Hyphal Formation, and Virulence.  Journal of Microbiology 51: 345-351.  (June).   SCI.

Jiun-Nong Lin, Chih-Chao Lin, Chung-Hsu Lai, Yun-Liang Yang, Hui-Ting Chen, Hui-Ching Weng, Li-Yun Hsieh, Yi-Chi Kuo, Tsai-Ling Lauderadale, Fan-Chen Tseng, Hsi-Hsun Lin, Hsiu-Jung Lo.  (2013).  Predisposing factors for oropharyngeal colonization of yeasts in human immunodeficiency virus-infected patients: A prospective cross-sectional study.  Journal of Microbiology, Immunology and Infection 46:129-135.  (April).   SCI.

Moan-Shane Tsai, Yun-Liang Yang, An-Huei Wang, Lih-Shinn Wang, Daniel C. T. Lu, Ci-Hong Liou, Li-Yun Hsieh, Chi-Jung Wu, Ming-Fang Cheng, Zhi-Yuan Shi, Hsiu-Jung Lo.  (2012).  Susceptibilities to Amphotericin B, Fluconazole and Voriconazole of Trichosporon Clinical Isolates.  Mycopathologia 174:121-130.  (August).  SCI.

Wu, Chi-Jung; Lee, Hsin-Chun; Yang, Yun-Liang; Chang, Chia-Ming; Chen, Hui-Ting; Lin, Chih-Chao; Yao, Lee; Chu, Wen-Li; Hsieh, Li-Yun; Wang, Ya-Li; Lauderdale, Tsai-Ling; Tseng, Fan-Chen; Ko, Nai-Ying; Ko, Wen-Chien; Lo, Hsiu-Jung.  (2012).  Oropharyngeal Yeast Colonization in HIV-infected Outpatients in Southern Taiwan: CD4 Count, Efavirenz Therapy and Intravenous Drug Use Matter.  Clinical Microbiology and Infection 18:485-90.  (May).  SCI.

Yun-Liang Yang, Chih-Chao Lin, Te-Pin Chang, Tsai-Ling Lauderdale, Hui-Ting Chen, Ching-Fu Lee, Chin-Wen Hsieh, Pei-Chen Chen, Hsiu-Jung Lo.  (2012).  Comparison of Human and Soil Candida tropicalis Isolates with Reduced Susceptibility to Fluconazole.  PLoS One 7 e34609.   (April).   SCI


Yun-Liang Yang, Chih-Wei Wang, Shiang Ning Leaw, Te-Pin Chang, I-Chin Wang, Chia-Geun Chen, Jen-Chung Fan, Kuo-Yun Tseng, Szu-Hsuan Huang, Chih-Yu Chen, Ting-Yin Hsiao, Chao Agnes Hsiung, Chiung-Tong Chen, Chwan-Deng Hsiao, and Hsiu-Jung Lo.  (2012).  R432 is a key residue for the multiple functions of Ndt80p in Candida albicans.  Cellular and Molecular Life Sciences.  69:1011-1023. (March).  SCI.

Yang,Yun-Liang, Hsieh, Li-Yun, Wang, An-Huei, Lo, Hsiu-Jung, TSARY Hospitals.   (2011).  Characterization of Candida Species from Different Populations in Taiwan.   Mycopathologia 172: 131-139 (August).  SCI

Chen, Yen-Ning, Lo, Hsiu-Jung, Wu, Chia-Chen, Ko, Hui-Ching, Chang, Te-Pin, and Yang, Yun-Liang*.   (2011).  Loss of Heterozygosity of FCY2 Leading to the Development of Flucytosine Resistance in Candida tropicalis.   Antimicrobial Agents and Chemotherapy 55:2506-2514 (June).  SCI

Yang, Y.-L., Leaw, S.-N., Wang, A.-H., Chen, H.-T., Cheng, W.-T., Lo, H.-J.   (2011).  Characterization of Yeasts Colonizing in Healthy Individuals.   Medical Mycology 49:103-106 (January).  SCI

Yang, Y.-L., Hung, C.-C., Wang, A.-H., Tseng, F.-C., Leaw, S.-N., Tseng, Y.-T., Su, C.-L., Chen, H.-T., Lauderdale, T.-L., Lo, H.-J.   (2010).  Oropharyngeal Colonization of HIV-infected Outpatients in Taiwan by Yeast Pathogens.   Journal of Clinical Microbiology 48:2606-2612 (July).  SCI

Yang, Y.-L., Cheng, M.-F., Wang, C.-W., Wang, A.-H., Cheng, W.-T., Lo, H.-J. TSARY Hospitals.   (2010).  The Distribution of Species and Susceptibility of Amphotericin B and Fluconazole of Yeast Pathogens Isolated from Sterile Sites in Taiwan.   Medical Mycology 48:328-334 (March).  SCI

Li, S.-Y., Yang, Y.-L., Lin, Y.-H., Ko, H.-C., Wang, A.-H., Chen, K.-W., Wang, C.-W., Chi, H., Lo, H.-J., TSARY Hospitals.   (2009).  Two Closely Related Fluconazole-resistant Candida tropicalis Clone Circulating in Taiwan from 1999 to 2006.   Microbial Drug Resistance 15:205-210 (Sept).  SCI

Chen, C.-G., Yang, Y.-L., Tseng, K.-Y., Shih, H.-I., Liou, C.-H., Lin, C.-C., Lo, H.-J.   (2009).  Rep1p Negatively Regulating MDR1 Efflux Pump Involved in Drug Resistance in Candida albicans.   Fungal Genetics and Biology 46:714-720 (Sept).  SCI

Chen, S.-H., Chuang, Y.-C., Lu, Y.-C., Lin, H.-C., Yang, Y.-L., Lin, C.-S.   (2009).  A Method of Layer-by-layer gold Nanoparticles Hybridization in a Quartz Crystal Microbalance DNA Sensing System Used to Detect Dengue Virus.   Nanotechnology 20:215501 (May).  SCI

Yang, Y.-L., Wang, C.-W., Chen, C.-T., Wang, M.-H., Hsiao, C.-F., Lo, H.-J.   (2009).  Non-lethal Candida albicans cph1/cph1 efg1/efg1 Mutant Partially Protects Mice from Systemic Infections by Lethal Wild-type Cells.   Mycological Research 113:388-390 (March) [changing title as Fungal Biology in 2010].  SCI

Yang, Y.-L., Cheng, M.-F., Chang, Y.-W., Young, T.-G., Chi, H., Lee, S.-C., Cheung, B. M.-H., Tseng, F.-C., Chen, T.-C., Ho, Y.-H. , Shi, Z.-Y., Chan, C.-H. H, Lin, J.-Y., and Lo, H.-J.   (2008).  Host Factors Do Not Influence the Colonization Or Infection by Fluconazole Resistant Candida Species in Hospitalized Patients.   Journal of Negative Results in BioMedicine 7:12 (Dec).  SCI

Yang, Y.-L., Wang, A.-H., Wang, C.-W., Cheng, W.-T., Li, S.-Y., Lo, H-J., TSARY Hospitals.   (2008).  Susceptibilities to Amphotericin B and Fluconazole of Candida Species in TSARY 2006.   Diagnostic Microbiology and Infectious Disease 61:175-180 (June).  SCI

Yang, J.-M., Chen, Y.-F., Tu, Y.-Y., Yen, K.-R., Yang, Y.-L.*   (2007).  Combinatorial Computation Approaches Identifying Tetracycline-derivatives as Inhibitors of Flaviviruses.   PLoS One 5:e428 (May).  SCI

Chiu, M.-W., Shih, H.-M., Yang, T.-H., Yang, Y.-L.*   (2007).  The Type 2 Dengue Virus Envelope Protein Interacts with Small Ubiquitin-like Modifier-1(SUMO-1) Conjugating Enzyme 9 (Ubc9).   J. Biomedical Science 14:429-444 (May).  SCI

Wang, J.-S., Li, S.-Y., Yang,Y.-L., Chou, H.-H., Lo, H.-J.   (2007).  Association between Fluconazole Susceptibility and Genetic Relatedness among Candida tropicalis Isolates in Taiwan.   J. Medical Microbiology 56:650-653 (May).  SCI

Lin, C.-Y., Ching, Y.-T., Yang, Y.-L.   (2007).  Automatic Method to Compare the Lanes in the Gel Electrophoresis Images.   IEEE Transactions on Information Technology in Biomedicine 11(2):179-89 (Mar).   SCI

Wang, J.-S., Yang, Y.-L., Wu, C.-J., Ouyang, K. J.,Tseng, K.-Y., Chen, C.-G., Wang, H., and Lo, H.-J.   (2006).  The DNA Binding Domain of CaNdt80p Is Required for Activating CDR1 Involved in Drug Resistance in Candida albicans.   J. Medical Microbiology 55, 1403-1411 (Oct).  SCI

Chen, C.-G., Yang, Y.-L., Cheng, H.-H., Su, C.-L., Huang, S.-F., Chen, C.-T., Liu, Y.-T., Su, I.-J., and Lo, H.-J.   (2006).  Non-lethal Candida albicans cph1/cph1 efg1/efg1 Transcription Factor Mutant Establishing Restricted Zone of Infection in a Mouse Model of Systemic Infection.   International Journal of Immunopathology and Pharmacology 19:561-565 (Sept).  SCI

Yang, Y.-L., Lin, Y.-H., Tsao, M.-Y., Chen, C.-G., Shih, H.-I., Fan, J.-C., Wang, J.-S., and Lo, H.-J.   (2006).  Serum Repressing Efflux Pump CDR1 in Candida albicans.   BMC Molecular Biology 7:22 (July).  SCI

Yang, Y.-L., Cheng, H.-H., Lo, H.-J. TSARY Hospitals.   (2006).  Distribution and Antifungal Susceptibility of Candida albicans Species Isolated from Different Age Populations in Taiwan.   Medical Mycology 44:237-242 (May).  SCI

Yang,Y.-L.*, Chen, H.-F, Kuo, T.-J., Lin, C.-Y.   (2006).  Mutations on CaENO1 in Candida albicans Inhibit Cell Growth in the Presence of Glucose.   J. Biomedical Science: 13:312-21 (May).  SCI

Li, S.-Y., Yang, Y.-L., Chen, K.-W., Cheng, H.-H., Lauderdale, T.-L., Chiou, C.-S., Wang, T.-H., Hung, C.-C., and Lo, H.-J.   (2006).  Molecular Epidemiology of Long-term Colonization of Candida albicans Strains from HIV-infected Patients.   Epidemiology and Infection 134(2): 265-9 (April).  SCI

Yang, Y.-L.* , Lo, H.-J., Hung, C.-C., Li, Y.  (2006).  Effect of Prolonged HAART on Oral Colonization with Candida and Candidiasis.   BMC Infectious Diseases 6: 8 (Jan).  SCI

Yang, Y.-L., Li, S.-Y., Cheng, H.-H., Lo, H.-J. TSARY Hospital.  (2005).  The Trend of Susceptibilities to Amphotericin B and Fluconazole of Candida Species from 1999 to 2002 in Taiwan.  BMC Infect. Dis. 5:99.  (Nov). SCI

Lo, H.-J., Wang, J.-S., Lin, C.-Y., Chen, C.-G., Hsaio, T.-Y., Hsu, C.-T., Su, C.-L., Fann, M.-J., Ching, Y.-T., and Yang, Y.-L.*  (2005).  Efg1 Involved in Drug Resistance through Regulating the Expression of ERG3 in Candida albicans.  Antimicrob. Agents Chemo. 49: 1213-1215. SCI

Yang, Y.-L., Li, S.-Y., Cheng, H.-H., Lo, H.-J., TSARY Hospitals.  (2005).  Susceptibilities to Amphotericin B and Fluconazole of Candida Species in TSARY 2002.    Diagn. Microbial. Infect. Dis. 51: 179-183. SCI

Yang, Y.-L., Ho, Y.-A., Cheng, H.-H., Lo, H.-J., and TSARY Hospitals.  (2005).  Distribution and Susceptibility to Amphotericin B and Fluconazole of Candida Species Isolated from Taiwan.  Epidemiology and Infection. 133: 325-330. SCI

Huang, C.-C., Yang,Y.-L., Lauderdale, T. L., McDonald, L. C., Hsiao, C. F., Cheng, H. H., Ho, Y. A., Lo, H. J.  (2005).  Colonization of HIV-infected Outpatients in Taiwan with Candida species.  J. Clin. Microbiol. 43: 1600-1603. SCI

Cheng, M.-F., Yang, Y.-L., Yao, T.-J., Lin, C.-Y., Liu, J.-S., Tang, R.-B., Yu, K.-W., Fan, Y.-H., Hsieh, K.-S., Ho, M., Lo, H.-J.  (2005).  Risk factors for fatal candidemia caused by Candida albicans and non-albicans Candida species.  BMC Infect Dis.  5 (1): 22.  SCI

Yang, Y.-L., Lauderdale, T.-L., and Lo, H.-J.  (2004).  Molecular Mechanisms of Fluoroquinolone Resistance in Klebsiella.   Current Drug Targets-Infectious Disorders 4: 295-302.

Chen, C.-G.., Yang, Y.-L., Hsih, H.-I., Su, C.-L., and Lo, H.-J.  (2004).  CaNdt80 Is Involved in Drug Resistance in Candida albicans by Regulating CDR1.  Antimicrob. Agents Chemo.  48: 4505-4512. SCI

Yang,Y.-L., Cheng, H.-H., and Lo, H.-J.  (2004).  In vitro Activity of Voriconazole Against Candida Species Isolated in Taiwan.  Int. J. Antimicrob. Agents. 24: 294-296. SCI

Yang, Y.-L., Ho, Y.-A., Cheng, H.-H., Ho, M., and Lo, H.-J.  (2004).  Susceptibilities of Candida species to amphotericin B and fluconazole: The Emergence of Fluconazole Resistance in Candida tropicalis.  Infect. Con. Hosp. Epi. 25 (1):60-64. SCI

Cheng, M.-F., Tang, R.-B., Yu, K.-W., Fan, Y.-H., Yang, Y.-L., Hsieh, K.-S., Ho, M., and Lo, H.-J.  (2004). Distribution and Antifungal Susceptibility of Candida Species Causing Candidemia: Having Past History of Bacterial Infections Is a Risk Factor for Patients Infected with Fluconazole Less Susceptible Candida Species.  Diagn. Microbial. Infect. Dis. 48: 33-37. SCI

Chiu, M.-W. and Yang, Y.-L.*  (2003).  Blocking the Dengue Virus 2 Infections on BHK-21 Cells with Purified Recombinant Dengue Virus 2 E Protein Expressed in Escherichia coli.  Biochem. Bioph. Res. Co. 26;309(3):672-8.   SCI

Yang, Y.-L., Ho, Y.-A., Cheng, H.-H., Hsaio, C.-F., and Lo, H.-J.  (2003).  Fluconazole Resistance Rate in Candida Species from Different Region and Type of Hospitals in Taiwan.  J. Microbiol. Immunol. Infect. 36(3):187-91.  SCI.

 Yang, Y.-L.*  (2003)  Virulence Factors of Candida Species.  J. Microbiol. Immunol. Infect.  36:223-228.  SCI

Yang, Y.-L. and Lo, H.-J.  (2001).  Mechanisms of Anti-fungal Agent Resistance.  J. Microbiol. Immunol. Infect. 34:79-86.  SCI.


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