Pyrogram SNP Miner:
Using PyrosequencingTM to identify de novo SNPs in pooled DNA samples

Institute of Bioinformatics and Systems Biology
National Chiao Tung University
Hsinchu, Taiwan
Pyrogram SNP Miner About PSM Pyrosequencing Contact Lin Lab
The object of our method is using a pyrogram of a pooled DNA sample to estimate the frequency of the mutant allele in the sample and predict its sequence. The sequence and pyrogram from the wild type allele have to be known in advance.

Paste the dispensing order of dNTPs and the obtained pyrograms into the fields below.
Here is the example used in Lin et al. (2011). These data were copied from the txt output file generated by PyroMark Q96 ID.


The Pyrosequencing dispensing order of dNTPs:
(based on the sequence of the wild type DNA fragment)


Pyrogram of the wild type DNA fragment:


Pyrogram of the query (pooled) DNA sample:


The sliding window size:
The cutoff p-value for the Shapiro-Wilk normality test:

Pyrogram SNP Miner About PSM Pyrosequencing Contact Lin Lab